Введение

r-n-j

Российский неврологический журнал

Russian neurological journal

2658-79472686-7192

МИА

10.30629/2658-7947-2022-27-5-51-58

r-n-j-360

Research Article

ИССЛЕДОВАНИЯ И КЛИНИЧЕСКИЕ НАБЛЮДЕНИЯ

CLINICAL RESEARCHES AND CASE REPORTS

Показатели системы цитокинов и неоангиогенеза в крови при острой дискогенной пояснично-крестцовой радикулопатии у пациентов молодого возраста

Cytokines and neoangiogenesis parameters in young patients with acute discogenic lumbosacral radiculopathy

https://orcid.org/0000-0002-7682-6672

Максимова

М Ю.

Maksimova

M. Yu.

Москва

Moscow

ncnmaximova@mail.ru

https://orcid.org/0000-0002-6756-5511

Котляр

Я. А.

Kotlyar

Y. А.

Москва

Moscow

doctor_kot12@mail.ru

https://orcid.org/0000-0001-9604-7775

Шабалина

А. А.

Shabalina

A. A.

Москва

Moscow

ashabalina@yandex.ru

ФГБНУ «Научный центр неврологии»РоссияResearch Center of NeurologyRussian Federation

2022

24112022

2755158

2022

Максимова М.Ю., Котляр Я.А., Шабалина А.А.

Maksimova M.Y., Kotlyar Y.А., Shabalina A.A.

Данная работа распространяется под лицензией Creative Commons Attribution 4.0.

This work is licensed under a Creative Commons Attribution 4.0 License.

https://www.r-n-j.com/jour/article/view/360

Введение

Введение. Патогенез дискогенной пояснично-крестцовой боли у лиц молодого возраста является сложным и многокомпонентным. Данные по содержанию цитокинов и факторов неоангиогенеза при острой дискогенной пояснично-крестцовой радикулопатии немногочисленны и зачастую противоречивы.

Цель исследования

Цель исследования: оценить показатели системы цитокинов и неоангиогенеза в крови при острой дискогенной пояснично-крестцовой радикулопатии у пациентов молодого возраста.

Материал и методы

Материал и методы. В исследовании приняли участие 49 пациентов (27 (55,1%) мужчин и 22 (44,9%) женщины) в возрасте 36 [27; 45] лет с острой пояснично-крестцовой болью, обусловленной дегенеративными изменениями позвоночника и компрессией спинномозговых нервов по данным МРТ. Группу контроля составили 17 здоровых лиц (10 (58,8%) мужчин и 7 (41,2%) женщин) в возрасте 33 [25; 41] лет. Уровень C-реактивного белка измеряли на автоматическом биохимическом анализаторе Konelab 30Iprime (ThermoFisher, Финляндия). Уровни интерлейкина-1β (ИЛ-1β), интерлейкина-6 (ИЛ-6), интерлейкина-8 (ИЛ-8), фактора некроза опухоли альфа (ФНО-α), фактора роста эндотелия cосудов А (ФРЭС-А) в крови определяли твердофазным иммуноферментным методом (ELISA) на плашечном ИФА-анализаторе Реал-бест (Россия) с использованием наборов реагентов Cloud-Clone Corp. (США, Китай).

Результаты

Результаты. У пациентов молодого возраста с острой дискогенной пояснично-крестцовой радикулопатией по сравнению с контрольной группой выявлено повышение уровней С-реактивного белка (11,2 [7,1; 15,3] против 4,2 [3,5; 4,9] мг/мл; р = 0,011), ФНО-α (23,1 [16,8; 29,5] против 9,7 [6,9; 12,5] пг/мл; р = 0,001), ИЛ-1β (4,7 [3,1; 6,3] против 3,2 [2,3;4,1] пг/мл; р = 0,041), ИЛ-6 (11,2 [6,1; 16,3] против 4,5 [3,1; 5,9] пг/мл; р = 0,007), ИЛ-8 (30,3 [21,9; 48,8] против 20,5 [8,5; 32,6] пг/мл; р = 0,023) и ФРЭС-А (318 [260; 570] против 168 [100; 240] пг/мл; р = 0,002).

Заключение

Заключение. Полученные результаты подтверждают значение провоспалительных факторов и показателей неоангиогенеза в развитии острой дискогенной пояснично-крестцовой радикулопатии у пациентов молодого возраста.

Introduction

Introduction. Discogenic lumbosacral pain in young people has a complex and multicomponent pathogenesis. Evidence of the content of cytokines and neoangiogenesis factors in patients with acute discogenic lumbosacral radiculopathy are deficiency and often contradictory. Objective: to evaluate the cytokine and neoangiogenesis parameters in the blood of young patients with acute discogenic lumbosacral radiculopathy.

Materials and methods

Materials and methods. The study involved 49 patients (27 (55.1%) men and 22 (44.9%) women) with a mean age of 36 [27; 45] years with acute lumbosacral pain caused by degenerative changes in the spine and signs of compression of the spinal nerves, according to MRI. The control group consisted of 17 healthy individuals (10 (58.8%) men and 7 (41.2%) women) with a mean age of 33 [25; 41] years. The level of C-reactive protein was measured by an automatic biochemical analyzer Konelab 30Iprime (ThermoFisher, Finland). The levels of interleukin-1β (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), tumor necrosis factor alpha (TNF-α), vascular endothelial growth factor A (VEGF-A) in blood were determined by enzyme-linked immunosorbent assay (ELISA) on a plate ELISA analyzer Real-best (Russia) using reagent kits Cloud-Clone Corp. (USA, China).

Results

Results. Patients with acute discogenic lumbosacral radiculopathy, compared with the control group, has an increase in the levels of C-reactive protein (11.2 [7.1; 15.3] vs. 4.2 [3.5; 4.9] mg/ml; р = 0.011), TNF-α (23.1 [16.8; 29.5] vs. 9.7 [6.9; 12,5] pg/ml; р = 0.001), IL-1β (4.7 [3.1; 6.3] vs. 3.2 [2.3; 4.1] pg/ml; р = 0.041), IL-6 (11.2 [6.1; 16.3] vs. 4.5 [3.1; 5.9] pg/ml; р = 0.007), IL-8 (30. [21.9; 48.8] vs. 20.5 [8.5; 32.6] pg/ml; р = 0.023) and VEGF-A (318 [260; 570] vs.168 [100; 240] pg/ml; р = 0.002).

Conclusion

Conclusion. The obtained results confirm the importance of pro-inflammatory factors and indicators of neoangiogenesis in the development of acute discogenic lumbosacral radiculopathy in young patients.

острая дискогенная пояснично-крестцовая радикулопатияцитокиныфактор роста эндотелия сосудов А

acute discogenic lumbosacral radiculopathycytokinesvascular endothelial growth factor A

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The authors declare that there are no conflicts of interest present.